Melatonin Regulates Proliferation of ADSCs and Differentiation of ADSCs to SCs in vitro
SUN Yingying, LIU Yumei, LIU Yongchang, LIU Weiqi, CHANG Chenhao, ZHANG Ziqiang*
The purpose of this study was to investigate the effect of MT (melatonin) on the differentiation of ADSCs (adipose mesenchymal stem cells) into SCs (Schwann cells). ADSCs were isolated from mouse inguinal fat pad and cultured to the third generation for adipogenic and osteogenic differentiation. Cell surface antigens were identified by flow cytometry. The third-generation ADSCs were incubated with MT at different concentrations for 24 h, and the proliferation activity of cells was detected by the CCK-8 method, and then the appropriate concentration of MT was determined. The experiment was divided into control group, 50 nmol/L MT group, neural induction fluid group, and 50 nmol/L MT+neural induction fluid group. After 11 days of culture, the expression of GFAP and S-100 on the surface of SCs was detected by qPCR and Western blot. The results showed that the proliferation activity of 50 and 100 nmol/L MT on ADSCs was significantly higher than that of other groups, and the proliferation effect of 50 nmol/L MT was better. The mRNA and protein expressions of GFAP and S-100 in the nerve induction fluid group and 50 nmol/L MT+nerve induction fluid group were significantly higher than those in the control group, while the mRNA and protein expressions of GFAP and S-100 in the 50 nmol/L MT nerve induction fluid group were significantly higher than those in nerve induction fluid group. In conclusion, 50 nmol/L MT can significantly promote the proliferation and differentiation of ADSCs in vitro.
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