Establishment of Multidrug-resistant Breast Cancer Cell Line MCF-7/MDR^a and Its Preliminary Analysis on the Biological Properties

Abstract: In this paper a line of breast cancer cell named MCF-7/MDR^a with multidrug-resistance was established， and its elementary biological properties including drug-resistance， cell cycle dynamics， exterior transformation， and intracellular drug accumulation and so on， were analyzed. Results showed that MCF-7/MDR^a was of good resistance to several chemotherapeutants for tumor. The IC₅₀ of MCF-7/MDR^a to ADM was 500 times higher than that of nature MCF-7， and its IC₅₀ to other chemotherapyagents was 6?5 times higher too. However， the drug resistant index of the cells could keep on over 200 times higher after culturing MCF-7/MDR^a in the medium (without ADM) for 150 days. The multiplication time of MCF-7/MDRa was similar to that of their nature cells， but the most cells concentrated in S-phase and the least cells in G₁-phase of the cell cycle. The multiplication time could be accelerated in evidence with culturing the cells in the medium (without ADM). The expression levels of P-gp， LRP and GSTp on the cells increased significantly except for ER. The level of intracellular ADM concentration of the cells remained high quantity even after 6-day culture in the medium (without ADM). The results indicated that the multidrug-resistant cell species (MCF-7/MDR^a) established in this study was of good biological characteristics， multidrug-resistance especially， which would be helpful to research on MDR mechanism of cancer cells.

CSTR: 32200.14.cjcb.2006.04.0021