Establishment and Biological Characteristic Analysis of Corneal Epithelial Cell Line from a Human Embryo

Abstract: In order to construct the stable supporter and work platform for corneal basic research， corneal epithelial cells were isolated and cultured from a human embryo acquired by legal way. After human embryo corneal epithelial cell line was established through many cell generations， its biological character was researched. The results showed that cultured cells had typical character of epithelial cells. The cells grew fast and its maximal subculture frequency to go down to the future generation was 2 days. The expressions of K19 and PCNA could be found in embryo corneal epithelial cells in every generation. The K19 expression located in cytoplasm while PCNA expression located in nucleus. The proportion of cells in S phase of cell cycle was approximately 11%~3%. The chromosome karyotype analysis manifested that the number and morphous of chromosomes were similar in every generation cells. Thus， the human embryo corneal epithelial cells was fit for establishing a relative stable cell line. The cultured cells had early stage character in differentiation and reproductive activity with strong and stable character. The good condition existed in the course of cell growth. Furthermore， the genetic character of this cell line showed relative stable.

CSTR: 32200.14.cjcb.2006.04.0020