Dynamic Characteristics of Lamin B during the Formation of PM-embryos Induced by 6-DMAP

Abstract: Our previous researches indicated that nuclear envelope reocurred after adding 6-DMAP when mouse embryos were inhibited by nocodazole in the metaphase of the first mitosis. The paternal and maternal genomes reconstructed male and female pronuclear-like nuclei respectively， rather than fusing into one single karyocyte. These embryos were designated as post-mitotic embryos (PM-embryos)， in which two special nuclei exist in zygotic cytoplasm. This study indicated that after being removed the inhibitor (6-DMAP and nocodazole)， the embryos without nuclear envelope reoccurrence could further develop to cleavage， while the PM-embryos could not after being cultured for 24 hours. Furthermore， we investigated the dynamic characteristics of lamin B by immunofluorescence during the nuclear envelope reconstruction of PM-embryos. The result showed that lamin B recruited to the chromosome gradually after 6-DMAP was added into the culture solution. Integrated Nuclear envelope formed about 3 hours later when the recruitment of lamin B reached the maximum level. The mechanism of the formation of the nuclear envelope induced by 6-DMAP is also discussed in this article.
    

CSTR: 32200.14.cjcb.2006.04.0018