Research Progress on Repair Mechanism of DNA Breakage Induced by CRISPR/Cas9 System
YANG Liyun, CHEN Lijiao, LI Shangang*
In recent years, CRISPR (clustered regularly interspaced short palindromic repeats) and its associated protein Cas9 mediated gene editing technology has been widely used in biology, basic medicine and other research fields. The animal disease model constructed by this technology has promoted the research progress of pathogenesis and gene therapy. Cas9 nuclease cleavage and subsequent HDR (homology-directed repair) or NHEJ (non-homologous end joining) achieved gene editing work together, but the low efficiency of HDR restricted its application. Therefore, the mechanism of NHEJ and HDR repair is very important to improve the accuracy and efficiency of gene editing. This review summarizes the mechanism of NHEJ and HDR repair system in the application of CRISPR/Cas9 system, and prospects the related research progress, in order to provide some ideas for the efficient use of CRISPR/Cas9 technology.
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