Protective Effect of Metformin on NMDA-Induced Injury in HT22 Cells
JIANG Mengmeng, LÜ Xin, ZHU Mengyao, YUAN Haiyang, GUO Zihe, WANG Yi, GONG Yuesong*
This study aimed to investigate the protective effect of Met on NMDA (N-methyl-D-aspartic acid)-induced HT22 cell damage. Firstly, a NMDA-induced HT22 cell injury model was established and pretreated with different concentrations of Met. Cell viability was detected to determine the optimal concentration of pre-protection of Met. Futhermore, Hoechst33342 staining was used to observe apoptotic cells. The activities of LDH, SOD and MDA in HT22 cells were detected by test kits. Finally, the expression levels of synaptic proteins PSD95, NR2B, AMPK and p-AMPK were detected by Western blot. The results showed that NMDA significantly caused HT22 cell damage compared with the control group, and 4 mmol/L Met showed maximum protection on HT22 cell damage induced by NMDA. NMDA increased apoptosis rate of HT22 cells, LDH activity, and MDA content, and decreased SOD activity, which was reversed following Met pretreatment. Met could also up-regulate the expression levels of PSD95, NR2B, and p-AMPK. In conclusion, Met has a neuroprotective effect by activating AMPK and modulating NR2B/PSD95 signaling pathway.
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