LINC00680 Regulates IL-17-Induced Proliferation, Migration and Invasion of Lung Cancer Cells by Targeting miR-195-5p
DI Yicheng1, SONG Yong2*, MA Xingqun3, WANG Zhaofeng2
This article mainly discusses the effects of LINC00680 targeted regulation of miR-195-5p on the IL-17-induced proliferation, migration and invasion of lung cancer cells. In this study, lung cancer cells H1299 were divided into Control group, IL-17 group, IL-17+si-NC group, IL-17+si-LINC00680 group, IL-17+siLINC00680+anti-miR-NC group, IL-17+si-LINC00680+anti-miR-195-5p group. Expression levels of LINC00680 and miR-195-5p were determined by qRT-PCR; cell proliferation was determined by clone formation experiments and MTT; cell migration and invasion abilities were measured by Transwell assay; Ki67, E-cadherin and N-cadherin protein expression levels were determined by Western blot; and the targeting relationship between LINC00680 and miR-195-5p was verified by dual-luciferase reporter system. Compared with the Control group, the relative expression of LINC00680, the number of clones, the cell viability, the number of migrating cells, the number of invasive cells, the expression of Ki67 and N-cadherin protein in the IL-17 group were significantly increased, and the expression of E-cadherin protein and the relative expression of miR-195-5p were significantly decreased. Compared with the IL-17+si-NC group, the relative expression of LINC00680, the number of cloned cells, cell viability, the number of migrating cells, the number of invasive cells, and the expression of Ki67 and N-cadherin protein in the IL-17+si-LINC00680 group were significantly decreased, but the expression of E-cadherin protein and the relative expression of miR-195-5p increased significantly. LINC00680 can targetedly regulate miR-195-5p expression, and the inhibition of miR-195-5p can reverse the effect of down-regulation of LINC00680 on IL-17-induced proliferation, migration and invasion of lung cancer cells. LINC00680 promotes the IL-17-induced lung cancer cells proliferation, migration and invasion through the regulation of miR-195-5p.
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