Isolation, Culture and Characterization of Gastric Mesenchymal Stem Cells from White Leghorns

QIN Xinxin^1,2*, SUN Nan^1,2, RUI Lei³, LI Zandong³

( ¹Ganzhou Key Laboratory of Basin Pollution Simulation and Control, Jiangxi University of Science and Technology, Ganzhou 341000, China; ²Jiangxi Provincial Key Laboratory of Environmental Geotechnology and Engineering Disaster Control, Jiangxi University of Science and Technology, Ganzhou 341000, China; ³State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China)

Abstract:

The aim of this study was to explore the growth characteristics of G-MSCs (gastric mesenchymal stem cells) isolated from 16-day-old White Leghorns, which provided methods for in vitro chicken research. Cell growth curve showed that G-MSCs possessed a good amplification ability in vitro. A better amplification ability occurred in P3 generation. The expression of cell surface markers was determined by RT-PCR (reverse transcription polymerase chain reaction). The CD29, CD44, CD73, CD71, CD90 and CD105 were positively expressed, while CD31, CD45 and CD34 were negatively expressed. Osteogenic induction experiment confirmed that G-MSCs could differentiate into osteogenic and adipogenic cells. Some osteogenic tissue blocks could be formed via a microsomal osteogenesis induction, with a low osteogenesis rate. An in vitro migration could also be observed from the target G-MSCs via cell scratch. The G-MSCs of White Leghorns were successfully isolated and identified, providing a basis for the further research on chicken G-MSCs and gastric cancer diseases treatment.

CSTR: 32200.14.cjcb.2021.12.0011