Effects of LncRNA LINC02310 on the Epithelial-Mesenchymal Transition and Radiosensitivity of Non-Small Cell Lung Cancer Cells
WU Chaohui*, YANG Jiansheng, LIN Xianbin, WU Jiayun, WU Jingyang
This study was to investigate the effects of LncRNA (long non-coding RNA) LINC02310 on EMT (epithelial-mesenchymal transition) and radiosensitivity of non-small cell lung cancer. qRT-PCR (real-time quantitative polymerase chain reaction) method was used to detect the relative expression of LINC02310 in nonsmall cell lung cancer tissues and cells (H1299, A549, H1650). H1299 cells were cultured in vitro, si-NC and siLINC02310 were transfected into the cells according to the liposome method, which were marked as si-NC group and si-LINC02310 group, and the relative expression of LINC02310 was detected by qRT-PCR; thiazole blue (MTT) method was used to detect cell proliferation; flow cytometry was used to detect cell apoptosis; Western blot method was used to detect Bax (Bcl-2 related X protein), Bcl2 (B-cell lymphoma/leukemia-2), Snail, E-cadherin, and Ncadherin protein expression; Transwell test was used to detect cell migration and invasion; clone formation test was used to detect radiosensitivity. Compared with adjacent tissues and normal human lung epithelial cells HBE, the relative expression of LINC02310 in non-small cell lung cancer tissues and cells was significantly increased. Compared with the si-NC group, down-regulating LINC02310 can reduce the D value, the number of migrated cells, the number of invaded cells, and the cell SF (survival fraction) of non-small cell lung cancer cells, increase the rate of apoptosis; reduce the expression of Bcl2, Snail, and N-cadherin, and increase the expression of Bax and E-cadherin. Downregulation of LINC02310 can inhibit non-small cell lung cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition, promote cell apoptosis and increase radiosensitivity.
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