The Effect of In Situ Injection of Human Umbilical Cord Mesenchymal Stem Cells on Premature Ovarian Failure in Rats
WU Yun1#, WANG Hao2#, WANG Zhe3, YU Hongliang2, DAI Hongjian3, WEI Quanwei3, SHI Fangxiong3, YAN Li2 *, HAN Linxiao4 *
POF (premature ovarian failure) is one of the principal causes of female infertility. Although its causes are complex, autoimmune deficiency must be involved. hUCMSCs (human umbilical cord mesenchymal stem cells) can be used for tissue regeneration and repair. Therefore, the present study was designed to determine the role of hUCMSCs in immune factor-induced POF in rats. In the present study, different concentrations of hUCMSCs were injected into induced POF rats. Ovarian functions were examined by evaluating the estrus cycle, follicular morphology, hormonal secretion, and the proliferation and apoptosis of granulosa cells. The results showed that the estrus cycle of rats returned to normal and follicular development was significantly improved after transplantation of hUCMSCs. In addition, serum concentrations of E2 (17-estradiol), P4 (progesterone), and AMH (anti-Müllerian hormone) increased significantly with treatment. Transplantation of hUCMSCs also reduced the apoptosis of granulosa cells and promoted the proliferation of granulosa cells. All of these improvements were dose dependent. Furthermore, the results of related gene expression showed that transplanted human hUCMSCs upregulated the expression of Bcl-2, AMH, and FSHR in the ovary of POF rats and downregulated the expression of caspase-3. Thus, these results further validated the potential mechanisms of hUCMSCs in promoting the release of cell growth factors and enhancing tissue regeneration and provided a theoretical basis for the clinical application of stem cells in the treatment of premature ovarian failure.
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