The Selection and Characterization of the Peptide Binding to EpCAM
JIA Chenshuang*, LI Xinyao, CHEN Lirong, YUAN Yue, GAO Qian, LU Zheng, XU Jie, SHU Zhan, WANG Yiwen, HOU Yingchun*
EpCAM (epithelial/endothelial cell adhesion molecule) is an important surface marker of CSC (cancer stem cell). After 5 rounds of biopanning, 47 clones were randomly selected from the last round of phage eluted library for amplification. Thirty positive phage clones were preliminarily identified by ELISA method, and sequentially sequenced. After sequence analysis, 5 consensus sequence groups were concluded. Subsequently, several cultured cancer cells were tested and identified by immunofluorescence assay to evaluate its affinity to CSC. Based on the molecular and cellular test results, R8 clone presents the best specificity and sensitivity for EpCAM binding, and its peptide sequence was named as ESP1. ESP1 is of important potential to be used for the development of cancer targeted chemotherapy delivery in future, and such delivery will have CSCs killed as more as possible that improves the therapy efficacy and reduces recurrence.
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