Effect of Porcine Induced Pluripotent Stem Cell-Derived Endothelial Cells on Hind-Limb Ischemia of Mice
YU Yang, LI Yimei, WEI Renyue, CHAI Mengjia, LIU Zhonghua*, ZHANG Yu*
In this study, the repairing ability of porcine iPSC-ECs (iPSC-derived endothelial cells) was studied by using mouse hind-limb ischemia model, in vivo imaging, HE staining, immunohistochemistry, TUNEL staining and Real time-PCR. The results showed that PKH26-labled iPSC-ECs decreased sharply at the early stage of cell transplantation, and the positive signal could seldom be observed after 7 days. Porcine iPSC-ECs treated group had organized morphology after 28 days of cell transplantation, and its total vessel density was (7.66±1.28)%, which was significantly higher than those of PFFs (porcine fetal fibroblasts) and EGM-2 treated groups. The apoptotic cell percentage of iPSC-ECs treated group was (25.83±2.32)%, which was significantly lower than those of the other two groups. In vitro study showed that, under hypoxic condition, the expression levels of pro-angiogenesis and anti-apoptotic genes of porcine iPSC-ECs were higher than those of PFFs, which was similar to porcine AOCs (aortic endothelial cells). These results suggest that porcine iPSC-ECs not only promote angiogenesis by fusion, but maybe play a role in anti-apoptosis and pro-angiogenesis by paracrine mechanism as well. Studies on porcine iPSCECs, on one hand, provide us experimental data for human endothelial cell transplantation, on the other hand, are beneficial for the establishment of cardiovascular drug screening platform and the understanding of endothelial differentiation and cardiovascular dysfunction.
CN
EN