The Effect of c-Jun Overexpression on the Function of Cord Blood Derived CD19.CAR-T cells

ZHANG Yao^1,2, LI Hua², ZHOU Dan³, MENG Lu³, LIU Qin³, ZHANG Tao^1,2*

(¹Medical College of Southwest Jiaotong University, Chengdu 610031, China; ²Western Theater General Hospital, Chengdu 610083, China; ³Chengdu Medical College, Chengdu 610500, China)

Abstract:

Antigen chimeric receptor-T cell therapy has shown significant curative effect on hematological malignancies, but the problem of tumor recurrence has not been solved. One of the reasons is that the expansion and activity of CAR-T cells in vivo are difficult to maintain. In the early stage, research group has successfully prepared CD19 targeted CAR-T cells derived from cord blood (CD19.CAR-CBT cells). To explore whether overexpression of c-Jun can effectively improve the expansion and durability of CAR-CBT cells, the study will exogenous c-Jun gene fragments and CD19.CAR gene into CBT cells to complete genetic modification. Prepare CD19.CAR-CBT cells overexpressing c-Jun transcription factor to study the effect of c-Jun overexpression on the activity of cord blood CAR-CBT cells.

CSTR: 32200.14.cjcb.2021.11.0001