Study on the Influence of Centrifugal Parameters on the Samples Sorted by Flow Cell Sorting
HUANG Yingying1*, XING Yueting1, MENG Xiaoyu2, SANG Wenhua2, LI Yanwei1, SONG Xinghui1, GUO Chun1, WANG Jiajia1
Flow cytometry is a kind of aseptic screening technology for cells or biological particles. Due to its advantages such as high speed, high flux, high sensitivity, etc, which has broad applications in medicine and life sciences. Centrifugation is a common technique for cell enrichment after flow cell sorting, which directly affects the cell viability and yield of samples sorted by flow cytometry. In this paper, based on FACS SORP Aria II cell sorter, KPC cell line (i.e. mouse pancreatic tissue-specific kras deletion p53 mutant cancer cells isolated from pancreatic
cancer, KPC) and mouse primary spleen cells are sorted. The experimental conditions such as centrifugal time and centrifugal force are optimized. The results show that KPC cells with high yield and viability can be obtained by centrifugation at 300 ×g, for 25 min at 4 °C, while for mouse primary spleen cells, centrifugation at 300 ×g, for 15 min at 4 °C is the most appropriate. This method can solve the problems of difficult centrifugation and large centrifugation loss, then improve the actual yield of cell sorting experiments and provide technical reference for the application of flow cytometry.
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