Fabrication and Evaluation of Decellularized Human Amniotic Membrane/Polycaprolactone Tissue Engineering Materials Implanted with Adipose-Derived Mesenchymal Stem Cells

In order to explore a new biomaterial for pelvic floor repair, a tissue engineering material with Co-ADSCs (co-culture adipose-derived mesenchymal stem cells) was constructed using dHAM (decellularized human amniotic membrane) as coat and PCL (polycaprolactone) as carrier. First, ADSCs (adipose-derived mesenchymal stem cells) were isolated and identified in vitro, and a co-culture system was established. Immunofluorescence, qRT-PCR and ELISA were used to detect the ability of Co-ADSCs to synthesize type I collagen. Meanwhile, dHAM was prepared and the degree of decellularization was evaluated. PCL film was prepared by solution casting method, and dHAM was composite on both sides of PCL film. The degradation test was carried out in vitro for 8 weeks. Finally, the cells were planted in dHAM/PCL, and the cytocompatibility and mechanical properties of the materials were evaluated by SEM, CCK-8 and tensile test. The results showed that adipogenic cells had the characteristics of mesenchymal stem cells, and ADSCs produced more type I collagen by co-culture. The prepared composite
was a “sandwich structure”, and the residual content reached 82.12% after 8 weeks of degradation. The dHAM/PCL cell compatibility was good, and the mechanical parameters of the materials were improved after Co-ADSCs transplantation. In conclusion, the tissue-engineered Co-ADSCs-dHAM/PCL composite biomaterial is degradable, cell-compatible and has good mechanical properties, so it is expected to be a new pelvic floor repair material.

CSTR: 32200.14.cjcb.2021.10.0007