Effects of Long Non-Coding RNA MALAT1 on Proliferation and Apoptosis of Diffuse Large B Cell Lymphoma
MA Le1, GONG Qiang1, LI Xi2, CHEN Jieping1*
This study was to investigate the role of long non-coding RNA MALAT1 in DLBCL (diffuse large B cell lymphoma) and the underlying molecular mechanism. DLBCL cell lines SU-DHL-1 and SU-DHL-4 were cultured in vitro, and MALAT1 was overexpressed or inhibited. The expression level of MALAT1 in each group was analyzed by real-time qPCR. The proliferation activities were analyzed by CCK-8 assay and BrdU staining assay. The apoptotic rates were detected by Annexin V/PI double staining assay, and the activation of β-catenin signaling pathway was analyzed by immunoblotting assay. Compared with Control group, overexpression of MALAT1, the expression of MALAT1 in DLBCL cells was significantly increased (P<0.001), cell proliferation activity was significantly increased (P<0.05), and the proportion of BrdU positive cells was significantly increased (P<0.01). Compared with the control siRNA transfection group, the expression level of MALAT1 and proliferation activity of cells in the MALAT1 siRNA transfection group were significantly decreased (P<0.01), and the proportion of BrdU positive cells was significantly decreased (P<0.001). The proportion of late apoptosis was significantly increased (P<0.001). Immunoimprinting results showed that overexpression of MALAT1 promoted the phosphorylation of GSK3β and the activation of β-catenin, while inhibition of MALAT1 decreased the phosphorylation of GSK3β and the activation of β-catenin. These results indicated that MALAT1 could promote the proliferation of DLBCL cells, inhibit apoptosis, and affect the activation of β catenin signaling pathway.
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