The Effect of circTLK1 on the Glomerular Mesangial Cells Injury Induced by High Glucose by Regulating the Expression of miR-374a-5p

To explore the effect of circTLK1 on the glomerular mesangial cells injury induced by high glucose and its molecular mechanism, this study divided the human glomerular mesangial cells HMCL into Con (control) group, HG (high glucose) group, HG+si-NC group, HG+si-circTLK1 group, HG+miR-NC group, HG+miR-374a-5p group, HG+si-circTLK1+anti-miR-NC group, HG+si-circTLK1+anti-miR-374a-5p group. RT-qPCR was used to detect the expressions of circTLK1 and miR-374a-5p; ELISA was used to detect TNF-α and IL-6 levels; MTT was used to detect cell proliferation; Western blot was used to detect protein expression; dual luciferase report experiment was used to verify targeting relationship between circTLK1 and miR 374a-5p. The results showed that in glomerular mesangial cells induced by high glucose, the expression levels of circTLK1, TNF-α, IL-6, CyclinD1 and the cell viability were increased, the expression of miR-374a-5p and p21 was decreased (P<0.05). Down-regulation of circTLK1 expression or over-expression of miR-374a-5p, the expression levels of TNF-α, IL-6, CyclinD1 in glomerular mesangial cells induced by high glucose and the cell viability was decreased, the expression of p21 was increased (P<0.05). circTLK1 targeted and regulated miR-374a-5p; inhibition of miR-374a-5p expression reversed the effect of down-regulation of circTLK1 expression on high glucose-induced glomerular mesangial cell damage. The study concluded that down-regulation of circTLK1 expression might inhibit high glucose-induced glomerular mesangial cells injury by up-regulating miR-374a-5p.

CSTR: 32200.14.cjcb.2021.09.0008