microRNA-21 Targets PDCD4/PTEN to Inhibit the Apoptosis of ADSCs in Rats

This study explored the effect of miR-21 on apoptosis of ADSCs in rats, and provided basis for improving the survival rate of ADSCs transplantation. The apoptosis model of ADSCs was established in vitro, and the expression of miR-21 was detected by qRT-PCR. Lipofectamine 2000 was used to transfect miR-21 mimics in ADSCs, and qRT-PCR was used to detect the transfection efficiency of miR-21 mimics. CCK-8 and Hoechst 33258 were used to detect the effect of miR-21 overexpression on the survival rate of ADSCs. Western blot was used to detect the expression of apoptosis-related proteins after the overexpression of miR-21 in ADSCs. After H₂O₂-induced apoptosis, the expression of miR-21 in ADSCs was significantly down-regulated. After rat ADSCs were transfected with miR-21 mimics, the expression of miR-21 in ADSCs was significantly increased by 7.4 times compared with the control group. Compared with the miR-21 scramble group, the survival rate of ADSCs was significantly increased after the overexpression of miR-21 mimics; the expressions of pro-apoptotic proteins Caspase-3 and Bax were significantly decreased, while the expression of anti-apoptotic protein Bcl-2 was significantly increased. Compared with the control group, the mRNA of PDCD4 and PTEN were not significantly changed after miR-21 overexpression, but the protein expressions of PDCD4 and PTEN were significantly down-regulated. After blocking the expression of PDCD4 and PTEN with PDCD4 siRNA and Phen, the expression of Caspase-3 and Bax were significantly decreased, while the expression of Bcl-2 was significantly increased. In this study, miR-21 can increase the inhibitory effect of ADSCs on apoptosis by targeting PDCD4/PTEN.

CSTR: 32200.14.cjcb.2021.09.0005