lncRNA CBR3-AS1 Affects the Proliferation, Migration, Invasion and Apoptosis of Gastric Cancer HGC-27 Cells by Regulating miR-145-5p
LI Haili1, GAO Peizhen2*, GUO Weidong1, ZHANG Huijie1
In order to explore the effect of lncRNA CBR3-AS1 targeting miR-145-5p on the malignant biological behavior of gastric cancer HGC-27 cells, RT-qPCR (real-time fluorescent quantitative PCR) was performed to examine the expression levels of CBR3-AS1 and miR-145-5p in cancerous tissues and adjacent tumors of 30 patients with gastric cancer. Then, the CBR3-AS1 interference expression vector plasmid, miR-145-5p mimic, miR-145-5p inhibitor and CBR3-AS1 interference expression vector plasmid were transfected into gastric cancer cell HGC-27; cell viability was detected by MTT (tetramethylazolium salt colorimetry); flow cytometry was used to detect cell apoptosis rate; Transwell method was used to detect the cells number of migration and invasion; dual luciferase report test was used to detect the targeting relationship between CBR3-AS1 and miR-145-5p. Experiment result showed that the expression level of CBR3-AS1 in gastric cancer tissue was higher than that in adjacent tissues, while the expression level of miR-145-5p was lower than that in adjacent tissues (P<0.05). Overexpression of miR-145-5p or inhibition of lncRNA CBR3-AS1 expression can reduce the activity of HGC-27 cells, the number of migration and invasion cells, and increase the apoptosis rate (P<0.05). lncRNA CBR3-AS1 targeted and regulated miR-145-5p. Down-regulation of miR-145-5p reversed the effect of inhibiting the expression of lncRNA CBR3-AS1 on HGC-27 cells. Therefore, inhibiting the expression of lncRNA CBR3-AS1 may inhibit the migration, invasion and proliferation of HGC-27 cells, and promote cell apoptosis by up-regulating miR-145-5p.
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