The Study of Mouse PU.1 and KLF7 Protein Interaction by FRET Technology
LI Wen, SHAO Shuli, ZHANG Zhenzhu, TAN Ming, JIN Zhao, LI Jinwei, SUN Yingning*
The expressions of PU.1 and KLF7 genes are significantly positively correlated in multiple tissues.
Both the two transcription factors inhibit adipogenesis through repressing PPARγ expression during preadipocyte differentiation. However, little is known about their interaction. To verify the relationship between PU.1 and KLF7 in preadipocytes, in the present study, recombinant overexpression vector pECFP-PU.1 and pEYFP-KLF7 were constructed and transfected into 3T3-L1 cell lines. Laser confocal microscope was used to observe their co-localization in the nuclei of 3T3-L1 cell lines. The FRET (fluorescence resonance energy transfer) analysis revealed a significant energy resonance transfer between PU.1 and KLF7. These results confirm that there is a direct interaction between PU.1 and KLF7, which provides a new idea for the mechanism of regulating adipogenesis.
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