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Genistein Inhibit Cathepsin K Expression Stimulated by IL-1a in Osteoclast-like Cells
Yun-Lin Wang*, Xiao-Qing Liu, Fei Yang, Han-Hua Yao, Hong-Bing Wang, Qin Xia, Huan-Li Zhu
Department of Gerontism, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract: The osteoclast-like cells (OCLs) isolated from tissue of human giant cell tumor of bone， were stimulated by IL-1a in the presence of genistein. Then the expression of cathepsin K (CK) was determined by RT-PCR and Western blot in OCLs. Compared with control group， the expressions of CK were up-regulated after stimulated by IL-1a in other groups (P<0.01). Genistein down-regulated CK gene expression stimulated by IL-1a at the transcription level stimulated in dose-dependent manner (P<0.01) and its effect was abrogated partly after treated with the estrogen receptor antagonist ICI 182.780. Genistein inhibit CK expression stimulated by IL-1a through estrogen receptor in OCLs. We found IL-1a stimulated the expression of CK in OCLs.
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