g-Irradiation Induced Mouse Lymphoma Cell Apoptosis Involved the NADPH Oxidase Activity

Abstract: Mouse Lymphoma cell 3SB (p53^+/+) and 1B1C4 (p53^-/-) were irradiated with ⁶⁰Co g-ray. Their apoptotic morphology was observed with microscope. Apoptotic assay results demonstrated that those two cell lines were quite differently to g-irradiation. 3SB was very sensitive， while 1B1C4 was anti-sensitive to g-irradiation. Both DNA ladder experiment and erythrosine B staining results confirmed this conclusion. The signal transduction molecular caspase-3 and p53 were also analyzed. The activated caspase-3 and p53 were found up-regulated by g-irradiation with dose pattern in 3SB cell. The caspase-3 mRNA differential expressions were also amplified between those two cell lines， but no difference was found. Then the activity of NADPH oxidase was checked in these two cells， the activity of NADPH oxidase was found only in apoptotic 3SB cell. In our research we found that NADPH-oxidase is another upstream signal molecular which involved in irradiation induced apoptosis.

CSTR: 32200.14.cjcb.2006.03.0022