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Construction of a LongSAGE Library of Mouse Endometrium during Implantation
Yi-Min Xu, Jun-Lin He, Xue-Qing Liu, Xiao-Yun Liu, Xue-Mei Chen, Ying-Xiong Wang*
School of Public Health, Chongqing University of Medical Sciences, Chongqing 400016, China
Abstract: According to the principle of LongSAGE library construction， we have constrcted the LongSAGE library through several procedures， such as total RNA isolation from mouse endometrium， cDNA synthesis， 130 bp ditag formation， amplification of ditags， isolation of the 34 bp ditags， concatemer formation， cloning the concatemers into pZErO®-1， transformation. It is verified that the positive ratio of constructed LongSAGE library is 100% and the length of inserting fragments is more than 400 bp. These results suggests that the LongSAGE library of mouse endometrium during implantation is successfully generated.
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