Expression of MicroRNA-129-5p in Serum of Sepsis Patients and Its Effect on Pulmonary Endothelial Cells
YUAN Jianghan, YUAN yuan, ZHOU Fachun*
This study was aimed to investigate the expression of miR-129-5p (microRNA-129-5p) in serum of sepsis patients and its effect on pulmonary endothelial cells. Blood samples from patients with sepsis were collected, RT-qPCR (real-time quantitative PCR) was used to detect the level of miR-129-5p and the relationship between its expression level and clinical characteristics of patients was analyzed. miR-129-5p was overexpressed in HPMEC (pulmonary microvascular endothelial cell), the effects of miR-129-5p on cell migration and the release of inflammatory cytokines were detected by Transwell and ELISA assay. RT-qPCR results showed that the expression level of miR-129-5p was decreased in the sepsis group compared with the control group (P<0.001), and the expression level of miR-129-5p in the septic shock group was lower than that in the sepsis group (P<0.05). The expression of miR-129-5p was positively correlated with oxygenation index and negatively correlated with PCT, IL-1β, IL-6 and TNF-α levels (P<0.001), and the 28-day mortality in the septic shock group was higher than that in the
sepsis group (P<0.05). Overexpression of miR-129-5p could significantly promote cell migration and reduce the release of inflammatory cytokines IL-1β, IL-6 and TNF-α. In conclusion, miR-129-5p was low expressed in the sepsis model. The more severe the disease was, the lower the level of miR-129-5p was. It was correlated with oxygenation index and PCT. Overexpression of miR-129-5p can significantly promote cell migration and reduce the release of inflammatory cytokines.
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