Incipient Establishment of Human Placenta Decidua Basalis-Derived Mesenchymal Stem Cells Bank
XIAO Guiqing1, DUAN Xunwei2, TANG Yancheng1, ZHENG Mengying1, SU Aimeng1, GENG Xiaobo1, DIAO Yong3*
The aim of this study was to explore the possibility of establishing the human placenta DBMSCs (decidua basalis-derived mesenchymal stem cells) bank as to provide an alternative source for the seed cells of tissue engineering. DB-MSCs were harvested from the decidua basalis of 10 cases of full-term human placental by tissue explants adherent method, and STR (short tandem repeat) test was used to identify the cells derived from the maternal placenta surface. The proliferative ability of the cells was detected by CCK8 assay. Flow cytometry was applied to analyze cell cycle and stem cell markers. The results of STR analysis showed that the cells were derived from the maternal placenta. The phenotype and expansion possibility of DB-MSCs after cryopreservation were remained. It could expand for 8 generations. The doubling time was (2.04±0.25) days. Cell cycle analysis showed majority cells stayed in the resting stage (G0/G1). The DB-MSCs kept stronger ability of adipogenesis, osteoblasts and chondrogenic differentiation. The bank of DB-MSCs has been incipiently established. This article further defines the inventory cell preparation process and its quality evaluation system which can provide eligible seed cells for tissue engineering.
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