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Mechanism of YAP Methylation Promoting Obesity

GU Xuemei¹^#, LI Zhiqiang²^#, GAO Renyuan², YU Haihong², ZHANG Chen², CAO Wenyue³, ZHANG Wen², NIU Wenhao⁴*, WANG Ping¹^,2*

(¹Shanghai Clinical College, Anhui Medical University, Hefei 230032, China; ²School of Medicine, Tongji University/Shanghai Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200092, China; ³Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China; ⁴Shanghai Changzheng Hospital, Shanghai 200001, China)

Abstract:

The increasing number of complications caused by obesity has a serious impact on people’s physical and mental health. The study found that YAP methylation could regulate fatty acid synthesis and oxidation to promote obesity. Yap K327M transgenic mice were used to demonstrate the relationship between YAP methylation and obesity, qRT-PCR was used to analyze the effect of YAP methylation on the expression of key genes in fatty acid synthesis and oxidation. The levels of glucose and growth factor in Yap K327M transgenic mice were analyzed by in vivo glucose and growth factor tolerance test, and the clinical samples were analyzed by immunohistochemical method. Data showed that YAP methylation promoted fatty acid synthesis and inhibited fatty acid oxidation. It can also suppressed glucose and growth factor tolerance, and then led to obesity in mice. Moreover, the level of YAP methylation in adipose tissue was significantly increased in obese patients. These results suggest that YAP methylation may be a potential marker for the diagnosis and prevention of obesity.

CSTR: 32200.14.cjcb.2021.06.0009