Comparative Study of Human Amniotic Fluid Derived Stem Cells and Bone Marrow Mesenchymal Cells

WANG Zeying¹, ZHANG Chenglong¹, SHEN Hongzhou¹, SI Jiawen¹*, SHI Jun¹, SHEN Guofang¹^,2*

(¹Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai 200011, China; ²Shanghai University of Medicine & Health Sciences, Shanghai 201318, China)

Abstract:

This work was to study the phenotype and osteogenic differentiation potential of hAFSCs (human amniotic fluid derived stem cells) and hBMSCs (human bone marrow mesenchymal stem cells). hAFSCs and hBMSCs were separated and cultured in vitro. Cellular phenotype was compared by light microscope, CCK-8 assessment, flow cytometry and gene-chip analysis. Then, the osteogenic capability of both cells was assessed with ALP (alkaline phosphatase) and ARS (alizarin red S) staining, Real-time PCR and cytoimmunofluorescence staining. Furthermore,
an ectopic osteogenic model of nude mice was used for estimating the in vivo osteogenesis capacity of both cells. Statistical analysis was performed using SPSS 16.0 software package. Both hAFSCs and hBMSCs showed spread spindle-like morphology, similar proliferation, and expression of CD90, CD105. ALP and ARS staining indicated the progressively increased ALP activity and mineralization under osteogenic induction, while the mRNA expression of RUNX2, OSX, COLI, ALP, OPN were also increased. Gene chip analysis indicated that there were differences in gene expression between hAFSCs and hBMSCs in terms of cell adhesion and inflammation. The in vivo study in nude mice demonstrated that hAFSCs and hBMSCs possessed similar differentiation potential of osteogenesis. In conclusion, hAFSCs and hBMSCs demonstrated similar morphology, proliferation and capacity of osteogenesis.

CSTR: 32200.14.cjcb.2021.06.0008