Construction and Application of Patient-Derived Organoids Model of Ovarian Cancer
HUANG Yuhan, LIU Chen, QIN Tianyu, SUN Chaoyang*, CHEN Gang*
This study aims to introduce a cost-effectiveness way to establish and culture a PDOs (patient-derived organoids) model of high grade serous ovarian cancer in detail. The results reveal the clinical value of PDOs model in choosing drugs for chemotherapy. To further extend the application of this model, techniques including comet assay, DNA fiber, immunofluorescence and Western blot are also successfully applied in PDOs models. By comparing the results of drug screening and clinical outcome after platinum treatment, a significant correlation of organoids and primary tumor tissue in drug sensitivity is found. In this article, PDOs model is verified to be necessary for exploring the best way to treat patients and promote the development of new drugs.
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