Research Advances of Organoids in Development and Regeneration
GUO Beibei, HU Huili*
Organoid is the in vitro three-dimensional culture system remodeling the structure and function of its original organ. Organoids have been widely used in developmental research, disease modeling and drug screening and have potential key application in regenerative medicine. Organoids derived from embryonic stem cells, induced pluripotent stem cells, and adult stem/progenitor cells from tissue recapitulate differentiation during development, selfrenewal in homeostasis, and regeneration after tissue injury, respectively. Organoids opened up new avenues to reveal the molecular mechanism of development and regeneration. It provides possibilities to clarify the physiological and pathological progression. Recently, scientists focus on establishing and characterizing new organoid models with multiple cell types by novel co-culture methods and new technology such as single-cell RNA sequencing, which facilitates the development of organoids. This review summarizes the latest research in development and regeneration using organoids. This review also gives an outlook for the application of cutting-edge technologies in organoid research.
CN
EN