lncRNA CCAT2 Affects Proliferation and Metastasis of NSCLC Cells by Activating Wnt/β-catenin Pathway via Regulating SIRT1
RUI Wenxiu*, WANG Liangge
lncRNA CCAT2 affects the proliferation and metastasis of NSCLC cells by regulating the expression of SIRT1 protein and activating Wnt/β-catenin signaling pathway. In this study, the expression of lncRNA CCAT2 in lung cancer tissues and lung cancer cell lines was detected by qRT-PCR. The relationship between lncRNA CCAT2 expression and clinicopathological features of lung cancer was analyzed by chi-square test. CCK-8 assay, scratch test and Transwell assay were used to observe the effect of lncRNA CCAT2 knockdown on the proliferation, migration and infiltration of lung cancer cells. After knocking down the expression of lncRNA CCAT2 expression in H1975 cell line, Western blot was used to detect the expression change of SIRT1 protein and Wnt/β-catenin signaling pathway. The effect of knockdown or overexpression of SIRT1 on Wnt/β-catenin signaling pathway were also detected. RNA immunoprecipitation and RNA pull-down experiments were used to verify the interaction between lncRNA CCAT2 and SIRT1. The results showed that the expression of lncRNA CCAT2 was significantly higher in cancer tissues and lung cancer cell lines (P<0.05). lncRNA CCAT2 knockdown inhibited the proliferation, migration and infiltration of H1975 cells. The expression of SIRT1, β-catenin, Cyclin D1, and myc proteins in H1975 cell line was decreased after the knockdown of lncRNA CCAT2. After knockdown of SIRT1, the expression of β-catenin, Cyclin D1 and myc proteins decreased. Compared with non-specific antibodies, SIRT1 antibodies were significantly enriched to lncRNA CCAT2. The expression of SIRT1 in truncated mutant cells of lncRNA CCAT2 was significantly lower than that of lncRNA CCAT2. lncRNA CCAT2 promotes lung cancer cell proliferation, migration and infiltration by activating Wnt/β-catenin signaling pathway via SIRT1, and lncRNA CCAT2 may become a new tumor marker.
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