Protective Effect of Telmisartan on Mice with Viral Myocarditis Induced by CVB3

YUAN Ling¹*, GAN Jihong², Zhi Ning³, GAO Yumei⁴, LIU Danyu⁵

(¹Outpatient Department of the Eighth Affiliated Hospital (North District) of Xinjiang Medical University, Urumqi 830014, China; ²Cardiology Department of General Hospital of Xinjiang Military Region, Urumqi 830000, China; ³Internal Medicine Department of the Eighth Affiliated Hospital (North District) of Xinjiang Medical University, Urumqi 830014, China; ⁴Medical Department of the Eighth Affiliated Hospital (North District) of Xinjiang Medical University, Urumqi 830014, China; ⁵Outpatient Department of the Eighth Affiliated Hospital of Xinjiang Medical University (Southern District), Urumqi 830049, China)

Abstract:

The aim of this study was to investigate the protective effect of telmisartan on CVB3 (Coxsackie B3)-induced viral myocarditis in mice. Sixty mice were randomly divided into control group, model group and observation group, with 20 mice in each group. The CVB3 virus was dissolved and injected into the abdominal cavity to make a model, and the observation group mice were fed with telmisartan for seven days and then sacrificed. This article observed the pathological conditions of the myocardial tissues of the three groups of mice, and detected the levels of SOD, MDA, GSH-Px, IL-1β, IFN-γ and TNF-α by enzyme-linked immunoassay in each group. The arrangement of myocardial cells in the observation group tended to be regular, with smaller gaps between cells and fewer inflammatory cells; the level of oxidative stress indicator MDA in the myocardial tissue of the model group was significantly higher than that of the normal group, while the levels of GSH-Px and SOD were significantly lower than those of the normal group (P<0.01); MDA level in the myocardial tissue of the observation group was significantly lower than that of the model group, while GSH-Px and SOD were significantly higher than that of the model group (P<0.01). The levels of IFN-γ, TNF-α and IL-1β in the myocardial tissue of the model group were significantly higher than those of the normal group (P<0.01), while the levels of inflammatory factors in the myocardial tissue of the observation group were significantly lower than those of the model group (P<0.01). The levels of iNOS, p-p65 and TLR4 in myocardial cells of the model group were significantly higher than those in the control group (P<0.01), while the levels of iNOS, p-p65, and TLR4 in the myocardial cells of the observation group were higher than those of the model group (P<0.01). The level of Nrf2-related protein in the cardiomyocytes of the model group was significantly lower than that of the control group (P<0.01), while the level of Nrf2-related protein in the cardiomyocytes of the observation group was lower than that of the model group (P<0.01). For the mice model of viral myocarditis, early use of telmisartan can reduce myocardial damage by participating in oxidative stress and inflammatory reaction process.

CSTR: 32200.14.cjcb.2021.05.0006