Expression of CHAF1A and ERα in Cervical Squamous Carcinoma and the Clinical Significance
WEN Li1, LI Xin2, JIA Ying1*
The study aims to investigate the expression and the relationship of CHAF1A (chromatin assembly factor 1 subunit) and ERα (estrogen receptor 1) in CSCC (cervical squamous cell carcinoma) tissues, as well as their clinical significance in the occurrence and development of CSCC. Immunohistochemical staining was used to detect the expression of CHAF1A and ERα in CSCC tissues and corresponding adjacent tissues. Total RNA was extracted from CSCC tissues and corresponding adjacent tissues, and mRNA levels were detected by RT-PCR to analyze their correlation. Western blot was used to detect the differences in the expression of CHAF1A and ERα in HeLa, C33a, CaSki, Siha and HaCat cells. Lentivirus-mediated ShRNA knocked down CHAF1A mRNA expression, and RT-PCR was used to detect the effect of CHAF1A knockdown on ERα expression. The results showed that the expression of CHAF1A increased with the development of CSCC lesions, and its expression in cervical cancer tissues was significantly higher than that in corresponding para-cancerous tissues, and its expression in cervical cancer cell lines was also higher than that in human normal epidermal cell lines. With the development of CSCC lesions, the expression of ERα was reduced, and the expression trend in cervical cancer tissues and cell lines was opposite to CHAF1A. The expression of ERα mRNA increased after CHAF1A gene knockdown. In addition, statistical analysis such as χ2 test, Spearman test and Student’s t test were used to explore the relationship between CHAF1A and ERα expression and the clinicopathological characteristics of CSCC. CHAF1A was highly expressed in cervical cancer and was positively correlated with the degree of CSCC malignancy; ERα was low in cervical cancer and was negatively correlated with the degree of CSCC malignancy, while the relation of ERα with CSCC was opposite. CHAF1A may have a regulatory effect on the expression of ERα.
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