Cell Wall Regeneration of Plant Protoplast

HE Qizouhong^1,2, E Yilan^1,2, WANG Guangchao^1,2, ZHANG Guifang¹, LIN Jinxing ^1,2, LI Ruili^1,2*

(¹Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing 100083, China; ²National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China)

Abstract:

Cell wall, as a significant component of plant cell, is essential for determining cell shape, maintaining mechanical support, and absorbing nutrients of plants. Thus, it is of great biological significance to reveal the regulatory mechanism of plant cell wall biosynthesis. Studying cell wall biosynthesis based on organizational levels has great limitations in terms of controlling the time scale and narrow observation space. Protoplast is an ideal model system for studying cell wall regeneration with cell wall removed. Over the past few decades, a large number of studies on cell wall regeneration from plant protoplast have been reported. However the mechanism underlying this process is yet unclear. This review introduces the primary techniques and research progresses applied to the study of plant cell wall regeneration in protoplast and unveils a prospective advances of this field, providing theoretical reference for further clarifying the mechanism of cell wall biosynthesis.

CSTR: 32200.14.cjcb.2021.01.0017