Correlation between Serum CCL2 and ANXA2 Levels and Invasive Growth of Cancer Cells in Patients with Gastric Cancer
WANG Wei, BULIBU·Jilisihan, XU Chunlei, SAIFUDING·Keyoumu*
This study is aimed to investigate the correlation between the contents of CCL2 (CC motif chemokine ligand 2) and ANXA2 (annexin A2) and the invasive growth of cancer cells in patients with gastric cancer, and to analyze their relationship with the clinicopathological characteristics of gastric cancer. With 187 gastric cancer patients as the research objects and 153 normal people as control group, ELISA (enzyme-linked immunosorbent assay) method was used to detect the serum levels of CCL2 and ANXA2 in all research objects. The clinicopathologic
features (cancer cell differentiation, invasion and lymph node metastasis) and the levels of CCL2 and ANXA2 among the groups were analyzed. The expression of Cripto-1, ZEB1, Snail, Vimentin, Piwil2, STOML2, BCL2, Smo, and XIAP in gastric cancer tissues and para-cancer tissues was detected by qRT-PCR, and the serum levels of CCL2 and ANXA2 were analyzed. Expression differences of Exosomes, Cripto-1, ZEB1, Snail, Vimentin, Piwil2, STOML2, BCL2, Smo and XIAP at different CCL2 and ANXA2 levels were analyzed. Immunohistochemical
SP staining was used to detect the levels of CCL2 and ANXA2 in all subjects and analyze the differences among groups. Serum levels of CCL2 and ANXA2 in gastric cancer group were significantly higher than those in control group (P<0.05). The gender, age, gastric cancer type and other data of the patients had no significant differences in CCL2 and ANXA2 levels (P>0.05). The degree of differentiation, the depth of infiltration and the lymph node metastasis showed significant differences in CCL2 and ANXA2 levels (P<0.05). The expression of Exosomes, Cripto-1, ZEB1, Snail, Vimentin, Piwil2, STOML2, BCL2, Smo and XIAP in high CCL2 and high ANXA2 groups were significantly higher than those in low CCL2 and low ANXA2 groups (P<0.05). Exosomes, Cripto-1, ZEB1, Snail, Vimentin, Piwil2, STOML2, BCL2, Smo and XIAP were significantly up-regulated in gastric cancer tissues compared with para-cancer tissues (P<0.05). According to the research results, the levels of serum CCL2 and ANXA2 are significantly correlated with the infiltration of cancer cells in gastric cancer patients, and the high expression of CCL2 and ANXA2 is closely related to the invasive growth and other biological behaviorse of gastric cancer cells.
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