Harmine Affects the Proliferation, Apoptosis, Migration and Invasion of Ovarian Cancer Cell CAOV3 through LOXL1-AS1
LI Heyue, ZHANG Zunsheng, WANG Jun*
This work was to explore the effect and molecular mechanism of harmine on the proliferation, apoptosis, migration and invasion of ovarian cancer cell CAOV3. Ovarian cancer cells CAOV3 were divided into control group, harmine low, medium, and high dose groups, si-NC group, si-LOXL1-AS1 group, harmine high dose+pcDNA group, harmine high dose+pcDNA-LOXL1-AS1 group. CCK-8 (cell counting kit-8) was uesd to detect cell viability; plate cloning test was uesd to detect the number of cell clones; flow cytometry was uesd to detect cell apoptosis; Western blot was uesd to detect protein expression; Transwell was uesd to detect cell migration and invasion; RT-qPCR (real-time fluorescent quantitative PCR) was uesd to detect the expression of LOXL1-AS1. The results showed that the cell survival rate of CAOV3 cells treated with different doses of harmine, the number of clone formation, cell migration and invasion, the expression of pro-caspase3 and LOXL1-AS1 were gradually decreased (P<0.05); the apoptosis rate and the expression of Cleaved-caspase3 were gradually increased. After interfering with the expression of LOXL1-AS1, cell survival rate, the number of clone formation, cell migration and invasion, the expression of pro-caspase3 were decreased (P<0.05); the cell apoptosis rate and the expression of Cleaved-caspase3 were increased. Overexpression of LOXL1-AS1 could attenuate the effects of harmine on the proliferation, apoptosis, migration and invasion of CAOV3 cells. This study showed that harmine could inhibit the proliferation, migration and invasion of ovarian cancer cell CAOV3 and promote apoptosis by down-regulating the expression of LOXL1-AS1.
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