Study on Self-Metabolism Alleviating Side-Effects of Methotrexate on the Mouse in-Vivo Matured Oocytes
LIU Meijun1, TIAN Ning1*, YU Ji1, MA Wanyun2*
MTX (methotrexate), an anti-folate drug, has been widely used in clinic. One of the side-effects of MTX is to reduce the quality of oocytes. So, how long it may take for the female patients with pregnancy needs to get pregnant after stopping taking MTX? In this paper, two-photon fluorescence imaging and three-dimensional reconstruction were combined to force on this issue. The model of mice in the control and MTX groups were established by intraperitoneally injecting physiological saline and 5 mg/kg MTX, and then the mice in the self-metabolism groups were established via metabolism lasting for five, 10, 15, 20, 25 and 30 days after MTX injection. It was found that the rate of normal spindle morphology and the rate of normal chromosome arrangement in the mouse invivo matured oocytes were significantly higher than those in the MTX group and returned to the levels of the control group after 15 and 25 days of metabolism, respectively. The in-vitro fertilization rate, two-cell embryo rate and blastocyst rate, which were used to characterized the function of oocytes, returned to the levels in the control group after 15, 20 and 25 days of metabolism, respectively. It indicated that self-metabolism could recover the MTX-induced quality impairment and functional decline of oocytes, and the recovery time was about five physiological cycles of mice, namely 25 days.
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