Establishment of a Highly Metastatic Murine Lymphoma Cell Model and Study on Their Biological Characteristics
CHEN Lirong1#*, JIA Yanmei1#, LI Yuanhong1, GUO Songjia2
This study established a highly metastatic murine lymphoma cell model. The malignant evolutionary cell subsets of mouse lymphoma cells were screened and their biological characteristics were studied. The biological characteristics and tumorigenicity of cell lines were examined by cell growth curve assay, chromosome karyotype analysis, spheroidization experiment in vitro, invasion and metastasis experiment and tumor formation experiment in vivo. The proliferative ability of LC2 was stronger than LC1, and the difference was significant. The population doubling time of LC1 cells was (24.33±2.12) h, and the population doubling time of LC2 cells was (20.52±2.71) h. Compared with LC1, the lymphocyte myeloid differentiation antigen marker
Gr1 was slightly increased, and the expression levels of stem cell markers Sca1 and CD44 were up-regulated in LC2. Both LC1 and LC2 were aneuploid karyotypes, and the aneuploid ratios of LC1 and LC2 were 5.24% and 89.12%, respectively. The clone formation ability of LC2 was significantly stronger than that of LC1 in different culture media. The migration and invasion abilities of LC2 were also significantly stronger than those of LC1. The LC2 had stronger tumorigenic ability in vivo than the LC1. This study found that LC1 cell line had a small number of metastatic foci in the kidney, but had no metastasis foci in the liver, while LC2 cell line had a 100% metastasis rate in the kidney and a large number of metastatic foci in the liver. This study successfully established a high metastatic mouse lymphoma cell model, which provided a good experimental material for studying the mechanism of lymphatic cancer high metastasis.
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