Comparison of Biological Characteristics of Human Synovial Mesenchymal Stem Cells and Human Umbilical Cord Mesenchymal Stem Cells

YAO Yingling¹,2, HE Ling¹^,2, BI Yang², NAN Guoxin¹^,2*

(¹Department of Orthopaedics Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China; ²Stem Cell Biology and Therapy Laboratory, Chongqing Key Laboratory of Pediatrics, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China)

Abstract:

The aim of this study was to investigate the differences in the biological characteristics of hSMSCs (human synovial mesenchymal stem cells) and hUC-MSCs (human umbilical cord mesenchymal stem cells). hUC-MSCs and hSMSCs were identified by flow cytometry. The proliferation, differentiation, migration abilities were comparatively studied between these two MSCs in vitro. The results showed that both hSMSCs and hUC-MSCs showed a long spindle-shaped cell morphology, and flow cytometry analysis showed that both hSMSCs and hUC-MSCs expressed cell surface markers characteristic of MSCs. hUC-MSCs showed high expression of CD73, CD105 and CD147, while hSMSCs showed high expression of CD24. Furthermore, hSMSCs showed more outstanding early osteogenesis and chondrogenesis differentiation abilities. Meanwhile, hUC-MSCs showed stronger proliferation and migration abilities, and the results of cell cycle showed that both hSMSCs and hUC-MSCs had strong proliferation ability. Our findings indicated that hSMSCs and hUC-MSCs presented some similar biological characteristics, and hSMSCs had stronger osteogenic and chondrogenic potentials, which might be used as ideal seed cells for tissue engineering or cells for clinical therapy.

CSTR: 32200.14.cjcb.2021.02.0004