The Effect of Lycium barbarum Soup on Oxidative Damage of Heroin to Retina
JIA Zongping1,2, GOU Taoran2, YU Shiyuan2*
The aim of this study was to explore the effects of Lycium barbarum soup on oxidative damage of heroin to retina. A total of 120 Kunming mice were randomly divided into control group, heroin group, L. barbarum 1 group and L. barbarum 2 group. The mice in heroin group, L. barbarum 1 group, and L. barbarum 2 group were intraperitoneally injected with increasing dose of heroin solution for 15 days (1-5, 6-10, and 11-15 days correspond to 2.0 g/L, 3.0 g/L, and 4.0 g/L heroin solution respectively, 0.2 mL each time, twice a day). In the control group, the same amount of normal saline was injected. After injecting heroin solution for 1 h, L. barbarum 1, 2 groups were given with 0.2 mL L. barbarum soup (L. barbarum 1 group and L. barbarum 2 group correspond to 0.2 g/mL and 0.4 g/mL L. barbarum soup respectively) by intragastric administration. The control group and heroin group were given with the same amount of normal saline. The changes of body mass and eyeball weight of the mice were measured. The changes of GSH content and LDH activity in plasma, SOD activity and MDA content in retina were detected by colorimetry, and the levels of Bax and TNF-α proteins in retina were detected by immunohistochemistry. The results showed that compared with the control group, the body mass and eyeball weight of the mice in heroin group decreased, the content of GSH in plasma decreased significantly, the LDH activity of plasma increased significantly, the activity of SOD in retina decreased, the content of MDA increased, the positive level of Bax and TNF-α protein in retina increased, and the average optical density increased significantly. Compared with the heroin group, the body mass and eyeball weight of the mice in L. barbarum groups increased again, the content of GSH in plasma increased, the LDH activity of plasma decreased significantly, the activity of SOD in retina increased, the content of MDA decreased, and the levels of Bax and TNF-α proteins in retina decreased again. In conclusion, L. barbarum soup can improve the anti-oxidation and anti-inflammatory abilities of retina cells and inhibit the positive levels of Bax and TNF-α proteins in retina, which has a certain protective effect on the retina.
CN
EN