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Rapid Detection of Anticancer Activity of Chinese Kale by Quinone Reductase Bioassay
Hai-Feng Zhang, Xun Xiang, Ping Wang, Qiao-Mei Wang*
Department of Horticulture, The State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development & Biotechnology, Zhejiang University, Hangzhou 310029, China
Abstract: Effect of different extract methods， lysing solutions， and the incubation time of quinone reductase (QR) reaction mixtures on bioassay was studied， and the results showed that the QR inducer potency of Chinese kale phosphate buffer (5 mmol/L K2HPO4-KH2PO4， 1 mmol/L EDTA， pH 7.6) extracts was comparable to the potency of common triple solvent extracts. Extraction with phosphate buffer was better than other methods extraction with organic solvents because of its simplicity and effectiveness. 0.4% Nonidet P-40 could be substituted for 0.8% digitonin during cell lysis in QR assay. The suitable incubation time of QR reaction mixtures in QR assay was 5 to 10 minutes.
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