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Triplont Analysis of Psendociaena crocea by Flow Cytometry
Chun-Jing Zheng*, Xiong-Fei Wu, Dong-Hai Liu1, Sun Zhang1
Ningbo Academy of Ocean and Fishery, Ningbo 315012, China; 1Ningbo No.2 Hospital, Ningbo 315010, China
Abstract: The authors try to establish the method for polysomaty analysis of Psendociaena crocea by flow cytometry. Psendociaena crocea fertilized eggs were shocked by cold or stress. After the chromosomes of gastrulae in the experimental groups were analysed. The triploid cells were sured in the experimental group. Then the group is cultivated to full-length 1-3cm fingers. And analysis of cells' chromosomes content of the fingers was made by flow cytometry. If the chromosomes content peak value of the finger in the experimental group is 1.5 in the control. This means the finger is triplont. The results show that 12.09% are triplonts of the 182 samples in the experimental groups. And 55.56% are triplonts in the one of experimental groups.
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