Expression of MMP-13 and HSP-90 in Hepatocellular Carcinoma and Their Clinicopathological Values

KOU Xuemei¹*, FEGN Xiangdong², CHEN Yi¹, CUI Fang¹, WAGN Xiaofeng¹, DONG Qiuyan³

(¹Department of Pathology, Beijing General Team Hospital of the Chinese People’s Armed Police Force, Beijing 100027, China; ²Department of Pathology, Beijing Fuxing Hospital Affiliated to Capital Medical University, Beijing 100027, China; ³Chinese people’s Armed Police Force Beijing General Hospital Ophthalmology, Beijing 100027, China)

Abstract:

This study was to investigate the expression of MMP-13 (matrix metalloproteinase-13) and HSP-90 (heat shock protein-90) in HCC (hepatocellular carcinoma) and their clinicopathological values. Totally 160 tissues of hepatocellular carcinoma and 160 tissues of non-hepatocellular carcinoma were collected. The expression levels of MMP-13, HSP-90α and HSP-90β were detected by immunohistochemistry, and the expression levels of MMP-16, HSP-90α and HSP-90β mRNA were measured by RT-PCR. The correlation between the expression of MMP-13, HSP-90α and HSP-90β and the clinicopathological characteristics of HCC were analyzed. Compared with non-HCC tissues, the positive rates of MMP-13, HSP-90α and HSP-90β in HCC tissues were significantly higher (P<0.05); in non-HCC tissues, the positive rates of MMP-13, HSP-90a and HSP-90β in hepatitis and cirrhosis tissues were significantly higher than those in normal liver tissues (P<0.05), while the positive rates of HSP-90α and HSP-90β in hepatitis and cirrhosis tissues were similar (P>0.05). There was a positive correlation between the expression of HSP-90α, HSP-90β and MMP-13 (r=0.527, 0.561, P<0.05). The positive rates of MMP-13, HSP-90α and HSP-90β in different pathological types and TNM stages of HCC tissues were not significantly different (P>0.05); the positive rates of MMP-13, HSP-90α and HSP-90β in differentiated hepatocellular carcinoma tissues were significantly different. The positive rates of MMP-13, HSP-90α and HSP-90β in poorly differentiated hepatocellular carcinoma tissues were significantly higher than those in moderately differentiated and highly differentiated hepatocellular carcinoma tissues (P<0.05). The positive rates of MMP-13, HSP-90α and HSP-90β in hepatocellular carcinoma with capsular involvement-portal vein cancer thrombus were significantly higher than those in hepatocellular carcinoma without capsular involvement-portal vein cancer thrombus (P<0.05). The expression levels of MMP-16, HSP-90α and HSP-90β mRNA in different differentiation degree and TNM stage HCC tissues were significantly different. The expression levels of MMP-16, HSP-90α and HSP-90β mRNA decreased with the increase of differentiation degree, and increased with the increase of TNM stage. The expression levels of MMP-16, HSP-90α and HSP-90β mRNA in HCC tissues with capsule involvement portal vein tumor thrombus were significantly higher than those without TNM stage capsule involvement-portal vein tumor thrombus (P<0.05). The expressions of HSP-90α, HSP-90β and MMP-13 in hepatocellular carcinoma tissues are significantly up-regulated, and they are closely related to clinicopathological features such as portal vein cancer thrombus and tissue differentiation.

CSTR: 32200.14.cjcb.2020.10.0009