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The Transferability of Chinese Cabbage EST-SSR Markers
Ya Xin1,2, Hai-Rui Cui1*, Ming-Long Zhang1, Yan-Ling Yao1, Mei-Zhen Lu1,Ji-Qiang Jin1,Yong-Pyo Lim3, Su-Ryun Choi3
1Institute of Nuclear and Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 2Biotechnology Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; 3Department of Horticultur
Abstract: EST-SSR is a new kind of SSR markers developed from expressed sequence tags (ESTs). The transferability of Chinese cabbage EST-SSR markers to different plant species was studied by designing 15 SSR primer pairs based on ESTs and using them for PCR to genomic DNA from Chinese cabbage， rapeseeds， rice， sorghum， maize and tea tree. The amplification percentage of designed primers to different varieties of Chinese cabbage， relatively related rapeseeds and distantly related rice， sorghum， maize and tea tree， were 100.0%， 93.3%， 80.0%， 93.3%， 93.3% and 86.7%， respectively. 11 of the 15 primer pairs showed the amplification in all distantly related species and the polymorphic primers account for 28.6%， 28.6%， 33.3% and 61.5% of available primers in rice， sorghum， maize and tea tree respectively. These results indicate that the Chinese cabbage EST-SSR marker is highly transferable to other plant species， being significant to studies on comparative genomics among different species.
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