The Repair Effect of Rutin and Anthocyanin on Renal Cells Damaged by High Uric Acid
LIU Jian, ZHOU Yuqiao, XU Mengjiao, YANG Yuqi, QU Daofeng*
In order to study the repair effect of rutin combined with anthocyanin on oxidative damage of renal cells, a uric acid-induced oxidative damage model of renal cells was established using HEK293 cell line. The activity of the damaged cells was determined by CCK-8 method. Hochest33342 (10 μg/mL) and PI (10 μg/mL) were used to stain the cells and observe their apoptosis. Finally, the change of intracellular ROS (reactive oxygen species) content was detected with the aid of the oxidative stress kit. The results showed that uric acid could induce oxidative damage of HEK293, and the optimal HEK293 cell damage model could be established by incubating the cells in a medium containing 1.4 g/L uric acid for 24 h. Both rutin and anthocyanin had certain repair effect on damaged cells, and the optimal repair effect (60% repair rate) was obtained when the two were used together (40 μg/mL rutin and 40 μg/mL anthocyanin). This study proves that the combined action of rutin and anthocyanin can play a better role in the repair of oxidative damaged renal cells.
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