Research Progress on the Construction and Application of Gastric Cancer Organoid

LI Tao¹, SONG Xiaoyu², CHU Fuhao³, LI Yuan¹, BO Rongqiang¹, YANG Ye¹, SU Zeqi4, DING Xia^3*

(¹Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China; ²Hefei National Science Center for Physical Sciences at Nanoscale, University of Science & Technology of China, Hefei 230027, China; ³School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China; ⁴Beijing Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China)

Abstract:

Gastric cancer is a common malignant tumor of digestive system with high morbidity and mor- tality, and poor prognosis. Currently, due to the lack of research models that mimic the characteristics of the occur- rence and development in gastric cancer, the researches on the pathogenesis and the development of therapeutic drugs are limited. Organoid is a kind of stem cell-derived three-dimensional cultured cell group with multicellular- assembly and self-renewal characteristics. The research model of gastric cancer organoids, can expand from genera- tion to generation, maintain the stability of the genome, possess the tumor heterogeneity and simulate tumor mi- croenvironment, to overcome the deficiencies of traditional two-dimensional cultured cell lines and animal models. This article recapitulates the establishment method of gastric cancer organoids and the latest progress in the study of gastric cancer pathogenesis, the simulation of the tumor microenvironment, the detection of gastric cancer related gene function, drug sensitivity screening and personalized treatment of gastric cancer. Furthermore, the advantages and disadvantages of organoids in the study of gastric cancer are discussed, in order to provide a model reference for revealing the pathogenesis of gastric cancer and developing drugs for the prevention and treatment of gastric cancer.

CSTR: 32200.14.cjcb.2020.09.0015