Role of miR-214 in the Repair of Cartilage Injury in Obese Mice
CAO Hong, WU Wei, ZHOU Xuchang, ZOU Jun, LIU Xiangyun, WANG Miao*
This study explored the mechanism of miR-214 in the cartilage repair in obese mice. At the age of five weeks, the mice were divided into control group (C group), obesity group (O group) and obesity+exercise group (OE group). C group was fed with conventional diet, and O group and OE group were fed with high-fat diet for 20 weeks. At the age of 17 weeks, the OE group was given with treadmill exercise for eight weeks, and all the samples were taken 48 h after the last intervention. The body mass was measured every week during the feeding period. Micro-CT scanning, paraffin section staining score and RT-PCR were performed. The results showed that the body masses of O group and OE group were significantly higher than that of C group, and the body mass of OE group decreased compared with O group during treadmill running. The staining scores of O group were significantly higher than those of C group, while Mankin’s score of OE group was significantly lower than that of O group. The BMD-related parameters of the medial tibial plateau in O group were significantly lower than those in C group, and Tb.N at the same site in OE group was significantly higher than that in O group. However, on the outside of the tibial plateau, only the BMD of O group was significantly lower than that of C group. Compared with C group, the expression of IL-6, ADAMTS5, and miR-214 were increased, while COL2 was decreased in O group. The expression of these genes in O group could be partially reversed in OE group after treadmill exercise. Studies have concluded that treadmill exercise can inhibit the expression of miR-214, thereby reducing the risk of cartilage damage and degeneration in obese mice, inhibiting the expression of inflammation, and promoting cartilage repair.
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