Resveratrol Treats Diabetes in Mice by Relieving Endoplasmic Reticulum Stress
QIN Dezhe, HE Chen, LI Balun, YANG Hong, HE Wenlai, SUN Jing, ZHANG Huimin, PENG Sha*
In this study, STZ (streptozotocin) was used to create a diabetic mouse model, which was modeled two weeks later. The mice were randomly divided into three groups, including 0 μmol resveratrol group (DD), 10 μmol resveratrol group (DR-10), and 100 μmol resveratrol group (DR-100). Resveratrol was given by intraperitoneal injection. HE and insulin immunofluorescence stainings of pancreatic tissue sections were performed to observe the changes in the volume and morphology of pancreatic islets. Using a blood glucose meter, the change of blood glucose was detected. Using qRT-PCR, the changes of endoplasmic reticulum stress-related genes in pancreatic tissues were measured. Western blot detected the changes of endoplasmic reticulum stress-related proteins. The results showed that after three weeks of intraperitoneal injection of resveratrol, the body masses of the model mice did not change significantly, the blood glucose levels of DR-10 and DR-100 groups decreased, and the amount of insulin secretion increased. Analysis of HE slices revealed that the cell damage in the pancreatic islets of DR-10 and DR-100 decreased. Immunofluorescence staining of mouse pancreas tissue sections revealed that the number of cells secreting insulin in DR-10 and DR-100 groups increased significantly. Resveratrol promotes the expression of insulin, glucagon, PDX1, C-Myc and Bcl-2, and reduces the expression of Grp78, CHOP and Caspase 3. In summary, resveratrol can alleviate the endoplasmic reticulum stress in the pancreas of diabetic model mice, thereby improving the symptoms of hyperglycemia in diabetic mice.
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