Cloning and Abiotic Stress Expression Analysis of BhMAPK15 Gene in Benincasa hispida (Thunb.) Cogn.
YE Xinru, WANG Bin, CHEN Mindong, LIU Jianting, ZENG Meijuan, ZHU Haisheng*, WEN Qingfang*
In order to learn the structure and the function of MAPK15 gene, the BhMAPK15 gene was cloned from Benincasa hispida (Thunb.) Cogn. by RT-PCR method based on transcriptome database. The sequence result showed that this gene contained an ORF (open reading frame) of 1 704 bp in length, which was predicted to encode a protein of 567 amino acid residues. Subcellular localization prediction indicated that BhMAPK15 may be located in both the cytoplasm and nucleus. Furthermore, BhMAPK15 gene family members were identified from plant species including model plant (Oryza sativa and Arabidopsis thaliana) and other different species. The paper characterized them based on sequence alignment, conserved domain and phylogeny. And then, homologous comparison and evolutionary analysis revealed that BhMAPK15 had the highest consistency with the MAPK15 of Cucumis melo, Cucurbita maxima, Cucumis sativus and Momordica charantia. The results of amino acid sequence and conserved motif analysis showed that the members of this gene family were more conserved. qRT-PCR analysis showed that the BhMAPK15 was expressed in all tested tissues, and its expression level could be upregulated by abiotic stress treatment. The above results indicate that the BhMAPK15 gene plays an important role in the response of abiotic stress.
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