Effects of TTK on Proliferation, Apoptosis, Invasion and Migration of Bladder Cancer Cells
LIU Jinyu1*, XIE Jianbing1, XIE Jinlai1, YAN Xiangshan1, LI Yongsheng2
The aim of this study was to explore the expression of TTK (threonine and tyrosine kinase) in bladder cancer tissues and the effect of TTK on the proliferation, apoptosis, invasion and migration of bladder cancer cells. qRT-PCR and immunohistochemistry were adopted to detect the expression of TTK mRNA and protein in paracancerous tissues, non-muscle-invasive bladder cancer tissues, and muscle-invasive bladder cancer tissues. TTK overexpression plasmid and TTK knockout plasmid were separately transfected into bladder cancer HT-1376 cells by lipofectamine. qRT-PCR and Western blot were used to detect the expression of TTK mRNA and protein after transfection; CCK and EdU assays were conducted to detect cell proliferation activity. Flow cytometry was used to detect cell cycle and apoptosis. The cell invasion and migration ability were determined by transwell assay. The results showed that the expression levels of TTK mRNA and protein in paracancerous tissues, non-muscleinvasive bladder cancer tissues, and muscle-invasive bladder cancer tissues gradually increased, and the differences among the three groups were statistically significant (P<0.05). After overexpressing TTK, the proliferation ability of HT-1376 cells was enhanced, the cell apoptosis rate was reduced, and the migration and invasion abilities of the cells were enhanced. After knocking out TTK, the proliferation of HT-1376 cells was reduced, the cell cycle was blocked at the G0/G1 phase, the cell apoptosis rate was increased, and the cell migration and invasion abilites were attenuated. The results of this study suggested that the expression of TTK is related to the progression of bladder cancer. TTK can promote the proliferation, invasion and migration of bladder cancer HT-1376 cells and induce cell apoptosis.
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