Exploratory Experimental Teaching Practice on Automatic Neural Tissue Dissociation to Viable Single Cells
SONG Xinghui1, SHEN Hao2, CHEN Jin1,2*
Single cell suspension preparation is a basic skill in Cell Biology. Commonly tissues are dissociated by mechanical and enzymatic methods. Different dissociation methods vary greatly in cell yield and cell viability. Neural single cell suspensions are in active use for a myriad of research applications, including the study of development, disease pathophysiology, drug and toxin screening, and grafting in animal models of neurological disorders. However, the acquisition of neural single cells is hampered due to the tight adhesion between neurons and the fragility of axons and dendrites. Automatic tissue dissociator combines the mechanical and enzymatic methods and can obtain high-quality cell suspensions. This technology is used to carry out a self-designed experiment of neural single cell suspension preparation, which is helpful to enhance students’ comprehensive analysis and application capabilities, and expand their understanding of cutting-edge science and technology. The integration of scientific research and teaching can better stimulate the learning enthusiasm of medical students, improve their scientific research quality, and achieve the goal of culticating research-oriented and innovative talents.
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